Recommendations From COVAS, Udgir Approved In Joint Animal Science Research Council Meet 2014
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Recommendation: 1 |
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| Title of the project: | Therapeutic management of Babesiosis in cattle |
| Investigators : | R. M., Shinde and A.U. Bhikane |
| Recommendation : Diminazene diaceturate @5 mg / kg IM with antoxidants Vit. E plus Selenium @2.5 ml/ 100 kg IM as an adjunct therapy is recommended for treatment of babesiosis in cattle . | |
| Recommendation: 2 | |
| Title of the project: | Development of Real Time PCR Protocol for rapid diagnosis of Brucellosis using DNA extracted directly from fetal abomasal contents and uterine discharge from aborted buffaloes |
| Investigators : | A.V Bhonsle, V.S Waskar, M.B Kulkarni, A.G Karpe, V.M Salunke & S.S Kulkarni |
| Recommendation : Real Time PCR protocol developed using DNA extracted directly from foetal abomasal contents and uterine discharge from aborted buffaloes can be employed as a rapid tool for diagnosis of brucellosis.शिफारस – म्हशींचा गर्भपात झाल्यानंतरत्वरीत गर्भाच्या पोटातील सामुग्री व म्हशीच्या गर्भाशयाच्यास्त्रावातून बृसिल्ला जीवाणूंची थेट मिळवलेली जनुकेवापरून विकसित वास्तविकवेळ (Real Time) PCR प्रोटोकॉल बृसिल्लोसीस (सांसर्गिकगर्भपात) च्या जलदनिदानासाठी उपयोजित केले जाऊ शकते. | |
Real Time PCR Amplification curves for SYBR Green omp gene B rucella melitensis serovar Abortus
Amplified omp gene from the DNA extracted directly from the clinical samples |
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Recommendation: 3 |
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| Title of the project: | Proteomic profiling of Brucella melitensis serovar Abortus isolated from aborted buffaloes from Marathwada Province in India |
| Investigators : | A.V Bhonsle, V.S Waskar, M.B Kulkarni, A.G Karpe & S.S Kulkarni |
| Recommendation: The proteomic profiling of Brucella melitensis serovar Abortus field isolates from Maharashtra province of India elucidated the protein profile ranging from 2424.983 to 19320.448 values, in addition to mass peaks ranging from 11212.860,16110.543, 19261.126 and 19320.448, corresponding to the protein mass peaks which can be useful in differentiating and rapid identification of Brucella melitensis serovar Abortus from field cases in India by MALDI TOF.शिफारस – भारतातील मराठवाडा प्रांतातून मिळविलेल्या Brucella melitensis serovar Abortus या जीवाणूची प्रथिने वापरून MALDI TOF द्वारे proteomic प्रोफाइलिंगकेल्यानंतर 2424.983 पासून19320.448 इतकेप्रथिनेवस्तुमानस्नीकपीकस्नोंदलेगेलेआहेत. अतिरिक्त वस्तुमान स्नीक पीकस्11212.860,16110.543, 19261,126 आणि19320,448 श्रेणीत नोंदवले आहेत. भारतातीलBrucella melitensis serovar Abortus जीवाणूंच्या जलद ओळखीसाठी सदरील proteomic प्रोफाइलिंग उपयोगी ठरू शकते. | |
Mass spectra (size, 2,000 to 11,000 Da) of Brucella melitensis field isolates grown under incubation at 37OC for 72 hours with 7% CO2. |
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Recommendation: 4 |
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| Title of the project: | Rapid MALDI TOF Protocol for identification of Escherichia coli and Proteus mirabilis isolated from Urinary tract infection in canines (6 Hrs protocol). |
| Investigators : | A.V Bhonsle, V.S Waskar, M.B Kulkarni, A.G Karpe, Premsagar & S.S Kulkarni |
| Recommendations: For rapid identification of Escherichia coli and Proteus mirabilis from canine urinary tract infection using MALDI TOF protocol (6 Hours), it is recommended to provide the short incubation to the urine sample to improve efficacy of MALDI-TOF analysis for >105 cfu/ml bacterial cells from the direct urine samples.शिफारस – श्वानवर्गीय प्राणांच्या मुत्रसंस्थेतील संसर्गातून मिळवलेल्या E Coli व Proteus mirabilis या जिवाणूंची शीघ्रओळख (> 105 CFU / मि.ली. जिवाणू पेशीसाठी) विकसित MALDI TOF प्रोटोकॉल(६ तास) विश्लेषणासह98% प्रभावीपणे उपयोजित केले जाऊ शकते. | |
Mass spectra (size, 2531 to 14,960 Da) of E coli field isolates grown under incubation at 37OC for 6 hours |
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Recommendation: 5 |
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| Title of the project: | Diagnosis and Surgical management of intestinal obstruction in bovines. |
| Investigators : | A.N. Meshram, C. L. Badgujar, K. S. Chaudhari, S. S. Pitlawar, A.U.Bhikane & M.A.Khan |
| Recommendations: Ultrasonography is recommended for the diagnosis of Intestinal obstruction or Intussuception at the ileum, caecum and colon parts of intestines. A Linear Transducer with the frequency from 5.0 to 7.5 MHz, for per rectal and a Curved Array Transducer with the frequency from 1.4 to 5.0 MHz for right flank, may be used, for this purpose. It gives confirmative diagnosis and additional information.शिफारस – गाई व म्हशींमध्ये लहान व मोठे आतडे यांच्या जोडणीच्या भागाजवळ होणारा अडथळा किंवा ‘एकात एक दुमडणे’ या आजाराच्या निदानासाठी अल्त्रासोनोग्राफी या तपासणीची शिफारस करण्यात येते. या कामासाठी गुद्दद्वाराच्या मार्गातून ५.० ते ७.५ MHz इतक्या फ़्रेक़्वेन्सीने लीनेअर प्रोब आणि उजव्या बाजूने बरगड्यांच्या मागील पाठीशी समांतर खळगा या भागावरून १.४ ते ५.० MHz इतक्या फ़्रेक़्वेन्सीने बहिर्वक्र प्रोब हे उपयुक्त आहेत. या तपासणी द्वारे वरील आजाराचे खात्रीशीर निदान करता येते व संबंधित अवयवांची पूरक माहिती मिळते. | |